An optimized protocol for microarray validation by quantitative PCR using amplified amino allyl labeled RNA

BACKGROUND: Validation of microarrays data by quantitative real-time PCR (qPCR) is often limited by the low amount of available RNA. This raised the possibility to perform validation experiments on the amplified amino allyl labeled RNA (AA-aRNA) leftover from microarrays. To test this possibility, w...

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Detalles Bibliográficos
Autores principales: Jeanty, Céline, Longrois, Dan, Mertes, Paul-Michel, Wagner, Daniel R, Devaux, Yvan
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3091691/
https://www.ncbi.nlm.nih.gov/pubmed/20929564
http://dx.doi.org/10.1186/1471-2164-11-542

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3091691/
https://www.ncbi.nlm.nih.gov/pubmed/20929564
http://dx.doi.org/10.1186/1471-2164-11-542

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