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Probing Carbohydrate Product Expulsion from a Processive Cellulase with Multiple Absolute Binding Free Energy Methods

Understanding the enzymatic mechanism that cellulases employ to degrade cellulose is critical to efforts to efficiently utilize plant biomass as a sustainable energy resource. A key component of cellulase action on cellulose is product inhibition from monosaccharide and disaccharides in the product...

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Detalles Bibliográficos
Autores principales: Bu, Lintao, Beckham, Gregg T., Shirts, Michael R., Nimlos, Mark R., Adney, William S., Himmel, Michael E., Crowley, Michael F.
Formato: Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093888/
https://www.ncbi.nlm.nih.gov/pubmed/21454590
http://dx.doi.org/10.1074/jbc.M110.212076