Peptide Labeling with Isobaric Tags Yields Higher Identification Rates Using iTRAQ 4-Plex Compared to TMT 6-Plex and iTRAQ 8-Plex on LTQ Orbitrap

Peptide labeling with isobaric tags has become a popular technique in quantitative shotgun proteomics. Using two different samples viz. a protein mixture and HeLa extracts, we show that three commercially available isobaric tags differ with regard to peptide identification rates: The number of ident...

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Detalles Bibliográficos
Autores principales: Pichler, Peter, Köcher, Thomas, Holzmann, Johann, Mazanek, Michael, Taus, Thomas, Ammerer, Gustav, Mechtler, Karl
Formato: Texto
Lenguaje:English
Publicado: American Chemical Society 2010
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093924/
https://www.ncbi.nlm.nih.gov/pubmed/20593797
http://dx.doi.org/10.1021/ac100890k

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093924/
https://www.ncbi.nlm.nih.gov/pubmed/20593797
http://dx.doi.org/10.1021/ac100890k

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