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Analysis of effects of tRNA:message stability on frameshift frequency at the Escherichia coli RF2 programmed frameshift site.
The codon that is in-frame prior to +1 frameshifting at the E.coli prfB (RF2 gene) frameshift site is randomized to create thirty-two variants. These alleles vary 1000-fold in frameshift-dependent expression in fusions to lacZ. Frameshifting is more frequent at sites where the in-frame codon ends in...
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
1993
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC309422/ https://www.ncbi.nlm.nih.gov/pubmed/8493101 |