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Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR

BACKGROUND: Reference genes are commonly used as the endogenous normalisation measure for the relative quantification of target genes. The appropriate application of quantitative real-time PCR (RT-qPCR), however, requires the use of reference genes whose level of expression is not affected by the te...

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Detalles Bibliográficos
Autores principales: Jarošová, Jana, Kundu, Jiban K
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3095291/
https://www.ncbi.nlm.nih.gov/pubmed/20630112
http://dx.doi.org/10.1186/1471-2229-10-146