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Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR
BACKGROUND: Reference genes are commonly used as the endogenous normalisation measure for the relative quantification of target genes. The appropriate application of quantitative real-time PCR (RT-qPCR), however, requires the use of reference genes whose level of expression is not affected by the te...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3095291/ https://www.ncbi.nlm.nih.gov/pubmed/20630112 http://dx.doi.org/10.1186/1471-2229-10-146 |