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Carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to HIV-gp140 antigen
Induction of humoral responses to HIV at mucosal compartments without inflammation is important for vaccine design. We developed charged wax nanoparticles that efficiently adsorb protein antigens and are internalized by DC in the absence of inflammation. HIV-gp140-adsorbed nanoparticles induced stro...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Elsevier Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098439/ https://www.ncbi.nlm.nih.gov/pubmed/21145913 http://dx.doi.org/10.1016/j.vaccine.2010.11.084 |
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author | Arias, Mauricio A. Loxley, Andrew Eatmon, Christy Van Roey, Griet Fairhurst, David Mitchnick, Mark Dash, Philip Cole, Tom Wegmann, Frank Sattentau, Quentin Shattock, Robin |
author_facet | Arias, Mauricio A. Loxley, Andrew Eatmon, Christy Van Roey, Griet Fairhurst, David Mitchnick, Mark Dash, Philip Cole, Tom Wegmann, Frank Sattentau, Quentin Shattock, Robin |
author_sort | Arias, Mauricio A. |
collection | PubMed |
description | Induction of humoral responses to HIV at mucosal compartments without inflammation is important for vaccine design. We developed charged wax nanoparticles that efficiently adsorb protein antigens and are internalized by DC in the absence of inflammation. HIV-gp140-adsorbed nanoparticles induced stronger in vitro T-cell proliferation responses than antigen alone. Such responses were greatly enhanced when antigen was co-adsorbed with TLR ligands. Immunogenicity studies in mice showed that intradermal vaccination with HIV-gp140 antigen-adsorbed nanoparticles induced high levels of specific IgG. Importantly, intranasal immunization with HIV-gp140-adsorbed nanoparticles greatly enhanced serum and vaginal IgG and IgA responses. Our results show that HIV-gp140-carrying wax nanoparticles can induce strong cellular/humoral immune responses without inflammation and may be of potential use as effective mucosal adjuvants for HIV vaccine candidates. |
format | Text |
id | pubmed-3098439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Elsevier Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30984392011-07-12 Carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to HIV-gp140 antigen Arias, Mauricio A. Loxley, Andrew Eatmon, Christy Van Roey, Griet Fairhurst, David Mitchnick, Mark Dash, Philip Cole, Tom Wegmann, Frank Sattentau, Quentin Shattock, Robin Vaccine Article Induction of humoral responses to HIV at mucosal compartments without inflammation is important for vaccine design. We developed charged wax nanoparticles that efficiently adsorb protein antigens and are internalized by DC in the absence of inflammation. HIV-gp140-adsorbed nanoparticles induced stronger in vitro T-cell proliferation responses than antigen alone. Such responses were greatly enhanced when antigen was co-adsorbed with TLR ligands. Immunogenicity studies in mice showed that intradermal vaccination with HIV-gp140 antigen-adsorbed nanoparticles induced high levels of specific IgG. Importantly, intranasal immunization with HIV-gp140-adsorbed nanoparticles greatly enhanced serum and vaginal IgG and IgA responses. Our results show that HIV-gp140-carrying wax nanoparticles can induce strong cellular/humoral immune responses without inflammation and may be of potential use as effective mucosal adjuvants for HIV vaccine candidates. Elsevier Science 2011-02-01 /pmc/articles/PMC3098439/ /pubmed/21145913 http://dx.doi.org/10.1016/j.vaccine.2010.11.084 Text en © 2011 Elsevier Ltd. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Article Arias, Mauricio A. Loxley, Andrew Eatmon, Christy Van Roey, Griet Fairhurst, David Mitchnick, Mark Dash, Philip Cole, Tom Wegmann, Frank Sattentau, Quentin Shattock, Robin Carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to HIV-gp140 antigen |
title | Carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to HIV-gp140 antigen |
title_full | Carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to HIV-gp140 antigen |
title_fullStr | Carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to HIV-gp140 antigen |
title_full_unstemmed | Carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to HIV-gp140 antigen |
title_short | Carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to HIV-gp140 antigen |
title_sort | carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to hiv-gp140 antigen |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098439/ https://www.ncbi.nlm.nih.gov/pubmed/21145913 http://dx.doi.org/10.1016/j.vaccine.2010.11.084 |
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