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Expression and Purification of Recombinant Hemoglobin in Escherichia coli

BACKGROUND: Recombinant DNA technologies have played a pivotal role in the elucidation of structure-function relationships in hemoglobin (Hb) and other globin proteins. Here we describe the development of a plasmid expression system to synthesize recombinant Hbs in Escherichia coli, and we describe...

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Detalles Bibliográficos
Autores principales: Natarajan, Chandrasekhar, Jiang, Xiaoben, Fago, Angela, Weber, Roy E., Moriyama, Hideaki, Storz, Jay F.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098879/
https://www.ncbi.nlm.nih.gov/pubmed/21625463
http://dx.doi.org/10.1371/journal.pone.0020176