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Expression and Purification of Recombinant Hemoglobin in Escherichia coli
BACKGROUND: Recombinant DNA technologies have played a pivotal role in the elucidation of structure-function relationships in hemoglobin (Hb) and other globin proteins. Here we describe the development of a plasmid expression system to synthesize recombinant Hbs in Escherichia coli, and we describe...
Autores principales: | Natarajan, Chandrasekhar, Jiang, Xiaoben, Fago, Angela, Weber, Roy E., Moriyama, Hideaki, Storz, Jay F. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098879/ https://www.ncbi.nlm.nih.gov/pubmed/21625463 http://dx.doi.org/10.1371/journal.pone.0020176 |
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