Cargando…

A Single Amino Acid Residue Constitutes the Third Dimerization Domain Essential for the Assembly and Function of the Tetrameric Polycystin-2 (TRPP2) Channel

Autosomal dominant polycystic kidney disease (ADPKD), the most common inherited cause of kidney failure, is caused by mutations in either PKD1 (85%) or PKD2 (15%). The PKD2 protein, polycystin-2 (PC2 or TRPP2), is a member of the transient receptor potential (TRP) superfamily and functions as a nons...

Descripción completa

Detalles Bibliográficos
Autores principales: Feng, Shuang, Rodat-Despoix, Lise, Delmas, Patrick, Ong, Albert C. M.
Formato: Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3099714/
https://www.ncbi.nlm.nih.gov/pubmed/21474446
http://dx.doi.org/10.1074/jbc.M110.192286
_version_ 1782204073784639488
author Feng, Shuang
Rodat-Despoix, Lise
Delmas, Patrick
Ong, Albert C. M.
author_facet Feng, Shuang
Rodat-Despoix, Lise
Delmas, Patrick
Ong, Albert C. M.
author_sort Feng, Shuang
collection PubMed
description Autosomal dominant polycystic kidney disease (ADPKD), the most common inherited cause of kidney failure, is caused by mutations in either PKD1 (85%) or PKD2 (15%). The PKD2 protein, polycystin-2 (PC2 or TRPP2), is a member of the transient receptor potential (TRP) superfamily and functions as a nonselective calcium channel. PC2 has been found to form oligomers in native tissues, suggesting that similar to other TRP channels, it may form functional homo- or heterotetramers with other TRP subunits. We have recently demonstrated that the homodimerization of PC2 is mediated by both N-terminal and C-terminal domains, and it is known that PC2 can heterodimerize with PC1, TRPC1, and TRPV4. In this paper, we report that a single cysteine residue, Cys(632), mutated in a known PKD2 pedigree, constitutes the third dimerization domain for PC2. PC2 truncation mutants lacking both N and C termini could still dimerize under nonreducing conditions. Mutation of Cys(632) alone abolished dimerization in these mutants, indicating that it was the critical residue mediating disulfide bond formation between PC2 monomers. Co-expression of C632A PC2 mutants with wild-type PC2 channels reduced ATP-sensitive endoplasmic reticulum Ca(2+) release in HEK293 cells. The combination of C632A and mutations disrupting the C-terminal coiled-coil domain (Val(846), Ile(853), Ile(860), Leu(867) or 4M) nearly abolished dimer formation and ATP-dependent Ca(2+) release. However, unlike the 4M PC2 mutant, a C632A mutant could still heterodimerize with polycystin-1 (PC1). Our results indicate that PC2 homodimerization is regulated by three distinct domains and that these events regulate formation of the tetrameric PC2 channel.
format Text
id pubmed-3099714
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher American Society for Biochemistry and Molecular Biology
record_format MEDLINE/PubMed
spelling pubmed-30997142011-05-27 A Single Amino Acid Residue Constitutes the Third Dimerization Domain Essential for the Assembly and Function of the Tetrameric Polycystin-2 (TRPP2) Channel Feng, Shuang Rodat-Despoix, Lise Delmas, Patrick Ong, Albert C. M. J Biol Chem Molecular Bases of Disease Autosomal dominant polycystic kidney disease (ADPKD), the most common inherited cause of kidney failure, is caused by mutations in either PKD1 (85%) or PKD2 (15%). The PKD2 protein, polycystin-2 (PC2 or TRPP2), is a member of the transient receptor potential (TRP) superfamily and functions as a nonselective calcium channel. PC2 has been found to form oligomers in native tissues, suggesting that similar to other TRP channels, it may form functional homo- or heterotetramers with other TRP subunits. We have recently demonstrated that the homodimerization of PC2 is mediated by both N-terminal and C-terminal domains, and it is known that PC2 can heterodimerize with PC1, TRPC1, and TRPV4. In this paper, we report that a single cysteine residue, Cys(632), mutated in a known PKD2 pedigree, constitutes the third dimerization domain for PC2. PC2 truncation mutants lacking both N and C termini could still dimerize under nonreducing conditions. Mutation of Cys(632) alone abolished dimerization in these mutants, indicating that it was the critical residue mediating disulfide bond formation between PC2 monomers. Co-expression of C632A PC2 mutants with wild-type PC2 channels reduced ATP-sensitive endoplasmic reticulum Ca(2+) release in HEK293 cells. The combination of C632A and mutations disrupting the C-terminal coiled-coil domain (Val(846), Ile(853), Ile(860), Leu(867) or 4M) nearly abolished dimer formation and ATP-dependent Ca(2+) release. However, unlike the 4M PC2 mutant, a C632A mutant could still heterodimerize with polycystin-1 (PC1). Our results indicate that PC2 homodimerization is regulated by three distinct domains and that these events regulate formation of the tetrameric PC2 channel. American Society for Biochemistry and Molecular Biology 2011-05-27 2011-04-07 /pmc/articles/PMC3099714/ /pubmed/21474446 http://dx.doi.org/10.1074/jbc.M110.192286 Text en © 2011 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access. Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) applies to Author Choice Articles
spellingShingle Molecular Bases of Disease
Feng, Shuang
Rodat-Despoix, Lise
Delmas, Patrick
Ong, Albert C. M.
A Single Amino Acid Residue Constitutes the Third Dimerization Domain Essential for the Assembly and Function of the Tetrameric Polycystin-2 (TRPP2) Channel
title A Single Amino Acid Residue Constitutes the Third Dimerization Domain Essential for the Assembly and Function of the Tetrameric Polycystin-2 (TRPP2) Channel
title_full A Single Amino Acid Residue Constitutes the Third Dimerization Domain Essential for the Assembly and Function of the Tetrameric Polycystin-2 (TRPP2) Channel
title_fullStr A Single Amino Acid Residue Constitutes the Third Dimerization Domain Essential for the Assembly and Function of the Tetrameric Polycystin-2 (TRPP2) Channel
title_full_unstemmed A Single Amino Acid Residue Constitutes the Third Dimerization Domain Essential for the Assembly and Function of the Tetrameric Polycystin-2 (TRPP2) Channel
title_short A Single Amino Acid Residue Constitutes the Third Dimerization Domain Essential for the Assembly and Function of the Tetrameric Polycystin-2 (TRPP2) Channel
title_sort single amino acid residue constitutes the third dimerization domain essential for the assembly and function of the tetrameric polycystin-2 (trpp2) channel
topic Molecular Bases of Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3099714/
https://www.ncbi.nlm.nih.gov/pubmed/21474446
http://dx.doi.org/10.1074/jbc.M110.192286
work_keys_str_mv AT fengshuang asingleaminoacidresidueconstitutesthethirddimerizationdomainessentialfortheassemblyandfunctionofthetetramericpolycystin2trpp2channel
AT rodatdespoixlise asingleaminoacidresidueconstitutesthethirddimerizationdomainessentialfortheassemblyandfunctionofthetetramericpolycystin2trpp2channel
AT delmaspatrick asingleaminoacidresidueconstitutesthethirddimerizationdomainessentialfortheassemblyandfunctionofthetetramericpolycystin2trpp2channel
AT ongalbertcm asingleaminoacidresidueconstitutesthethirddimerizationdomainessentialfortheassemblyandfunctionofthetetramericpolycystin2trpp2channel
AT fengshuang singleaminoacidresidueconstitutesthethirddimerizationdomainessentialfortheassemblyandfunctionofthetetramericpolycystin2trpp2channel
AT rodatdespoixlise singleaminoacidresidueconstitutesthethirddimerizationdomainessentialfortheassemblyandfunctionofthetetramericpolycystin2trpp2channel
AT delmaspatrick singleaminoacidresidueconstitutesthethirddimerizationdomainessentialfortheassemblyandfunctionofthetetramericpolycystin2trpp2channel
AT ongalbertcm singleaminoacidresidueconstitutesthethirddimerizationdomainessentialfortheassemblyandfunctionofthetetramericpolycystin2trpp2channel