Cargando…
Antiproliferative Effects of Cucurbitacin B in Breast Cancer Cells: Down-Regulation of the c-Myc/hTERT/Telomerase Pathway and Obstruction of the Cell Cycle
Naturally occurring cucurbitacins have been shown to have anticancer, antimicrobial and anti-inflammatory activities. In this study, we determined the effects of cucurbitacin B extracted from the Thai herb Trichosanthes cucumerina L. on telomerase regulation in three human breast cancer cell lines (...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100835/ https://www.ncbi.nlm.nih.gov/pubmed/21614210 http://dx.doi.org/10.3390/ijms11125323 |
_version_ | 1782204218587742208 |
---|---|
author | Duangmano, Suwit Dakeng, Sumana Jiratchariyakul, Weena Suksamrarn, Apichart Smith, Duncan R. Patmasiriwat, Pimpicha |
author_facet | Duangmano, Suwit Dakeng, Sumana Jiratchariyakul, Weena Suksamrarn, Apichart Smith, Duncan R. Patmasiriwat, Pimpicha |
author_sort | Duangmano, Suwit |
collection | PubMed |
description | Naturally occurring cucurbitacins have been shown to have anticancer, antimicrobial and anti-inflammatory activities. In this study, we determined the effects of cucurbitacin B extracted from the Thai herb Trichosanthes cucumerina L. on telomerase regulation in three human breast cancer cell lines (T47D, SKBR-3, and MCF-7) and a mammary epithelium cell line (HBL-100). Cell viability after treatment with cucurbitacin B, which is an active ingredient of this herb, was assessed. Telomeric Repeat Amplification Protocol (TRAP) assays and RT-PCR (qualitative and realtime) were performed to investigate activity of telomerase as well as expression of human telomerase reverse transcriptase (hTERT) and c-Myc. The c-Myc protein level was also determined in SKBR-3 and HBL-100 cells. Our results show that the cucurbitacin B inhibits growth and telomerase activity in the three breast cancer cell lines and exerts an obvious inhibitory effect in the estrogen receptor (ER)-negative breast cancer SKBR-3 cells. The expression of hTERT and c-Myc were also inhibited by cucurbitacin B, In addition, a clear reduction of c-Myc protein was observed after treatment in SKBR-3 cells even with a concentration of cucurbitacin B that was ten-times lower compared to the concentration used for HBL-100. Our findings imply that cucurbitacin B exerts an anticancer effect by inhibiting telomerase via down regulating both the hTERT and c-Myc expression in breast cancer cells. |
format | Text |
id | pubmed-3100835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-31008352011-05-25 Antiproliferative Effects of Cucurbitacin B in Breast Cancer Cells: Down-Regulation of the c-Myc/hTERT/Telomerase Pathway and Obstruction of the Cell Cycle Duangmano, Suwit Dakeng, Sumana Jiratchariyakul, Weena Suksamrarn, Apichart Smith, Duncan R. Patmasiriwat, Pimpicha Int J Mol Sci Article Naturally occurring cucurbitacins have been shown to have anticancer, antimicrobial and anti-inflammatory activities. In this study, we determined the effects of cucurbitacin B extracted from the Thai herb Trichosanthes cucumerina L. on telomerase regulation in three human breast cancer cell lines (T47D, SKBR-3, and MCF-7) and a mammary epithelium cell line (HBL-100). Cell viability after treatment with cucurbitacin B, which is an active ingredient of this herb, was assessed. Telomeric Repeat Amplification Protocol (TRAP) assays and RT-PCR (qualitative and realtime) were performed to investigate activity of telomerase as well as expression of human telomerase reverse transcriptase (hTERT) and c-Myc. The c-Myc protein level was also determined in SKBR-3 and HBL-100 cells. Our results show that the cucurbitacin B inhibits growth and telomerase activity in the three breast cancer cell lines and exerts an obvious inhibitory effect in the estrogen receptor (ER)-negative breast cancer SKBR-3 cells. The expression of hTERT and c-Myc were also inhibited by cucurbitacin B, In addition, a clear reduction of c-Myc protein was observed after treatment in SKBR-3 cells even with a concentration of cucurbitacin B that was ten-times lower compared to the concentration used for HBL-100. Our findings imply that cucurbitacin B exerts an anticancer effect by inhibiting telomerase via down regulating both the hTERT and c-Myc expression in breast cancer cells. Molecular Diversity Preservation International (MDPI) 2010-12-22 /pmc/articles/PMC3100835/ /pubmed/21614210 http://dx.doi.org/10.3390/ijms11125323 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Duangmano, Suwit Dakeng, Sumana Jiratchariyakul, Weena Suksamrarn, Apichart Smith, Duncan R. Patmasiriwat, Pimpicha Antiproliferative Effects of Cucurbitacin B in Breast Cancer Cells: Down-Regulation of the c-Myc/hTERT/Telomerase Pathway and Obstruction of the Cell Cycle |
title | Antiproliferative Effects of Cucurbitacin B in Breast Cancer Cells: Down-Regulation of the c-Myc/hTERT/Telomerase Pathway and Obstruction of the Cell Cycle |
title_full | Antiproliferative Effects of Cucurbitacin B in Breast Cancer Cells: Down-Regulation of the c-Myc/hTERT/Telomerase Pathway and Obstruction of the Cell Cycle |
title_fullStr | Antiproliferative Effects of Cucurbitacin B in Breast Cancer Cells: Down-Regulation of the c-Myc/hTERT/Telomerase Pathway and Obstruction of the Cell Cycle |
title_full_unstemmed | Antiproliferative Effects of Cucurbitacin B in Breast Cancer Cells: Down-Regulation of the c-Myc/hTERT/Telomerase Pathway and Obstruction of the Cell Cycle |
title_short | Antiproliferative Effects of Cucurbitacin B in Breast Cancer Cells: Down-Regulation of the c-Myc/hTERT/Telomerase Pathway and Obstruction of the Cell Cycle |
title_sort | antiproliferative effects of cucurbitacin b in breast cancer cells: down-regulation of the c-myc/htert/telomerase pathway and obstruction of the cell cycle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100835/ https://www.ncbi.nlm.nih.gov/pubmed/21614210 http://dx.doi.org/10.3390/ijms11125323 |
work_keys_str_mv | AT duangmanosuwit antiproliferativeeffectsofcucurbitacinbinbreastcancercellsdownregulationofthecmychterttelomerasepathwayandobstructionofthecellcycle AT dakengsumana antiproliferativeeffectsofcucurbitacinbinbreastcancercellsdownregulationofthecmychterttelomerasepathwayandobstructionofthecellcycle AT jiratchariyakulweena antiproliferativeeffectsofcucurbitacinbinbreastcancercellsdownregulationofthecmychterttelomerasepathwayandobstructionofthecellcycle AT suksamrarnapichart antiproliferativeeffectsofcucurbitacinbinbreastcancercellsdownregulationofthecmychterttelomerasepathwayandobstructionofthecellcycle AT smithduncanr antiproliferativeeffectsofcucurbitacinbinbreastcancercellsdownregulationofthecmychterttelomerasepathwayandobstructionofthecellcycle AT patmasiriwatpimpicha antiproliferativeeffectsofcucurbitacinbinbreastcancercellsdownregulationofthecmychterttelomerasepathwayandobstructionofthecellcycle |