MosSCI and Gateway Compatible Plasmid Toolkit for Constitutive and Inducible Expression of Transgenes in the C. elegans Germline

Here we describe a toolkit for the production of fluorescently tagged proteins in the C. elegans germline and early embryo using Mos1-mediated single copy insertion (MosSCI) transformation. We have generated promoter and 3′UTR fusions to sequences of different fluorescent proteins yielding construct...

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Detalles Bibliográficos
Autores principales: Zeiser, Eva, Frøkjær-Jensen, Christian, Jorgensen, Erik, Ahringer, Julie
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102689/
https://www.ncbi.nlm.nih.gov/pubmed/21637852
http://dx.doi.org/10.1371/journal.pone.0020082

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102689/
https://www.ncbi.nlm.nih.gov/pubmed/21637852
http://dx.doi.org/10.1371/journal.pone.0020082

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