An Efficient Strategy for Broad-Range Detection of Low Abundance Bacteria without DNA Decontamination of PCR Reagents

BACKGROUND: Bacterial DNA contamination in PCR reagents has been a long standing problem that hampers the adoption of broad-range PCR in clinical and applied microbiology, particularly in detection of low abundance bacteria. Although several DNA decontamination protocols have been reported, they all...

Descripción completa

Detalles Bibliográficos
Autores principales: Chang, Shy-Shin, Hsu, Hsung-Ling, Cheng, Ju-Chien, Tseng, Ching-Ping
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102696/
https://www.ncbi.nlm.nih.gov/pubmed/21637859
http://dx.doi.org/10.1371/journal.pone.0020303

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102696/
https://www.ncbi.nlm.nih.gov/pubmed/21637859
http://dx.doi.org/10.1371/journal.pone.0020303

Ejemplares similares