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Circular Permutation of Red Fluorescent Proteins

Circular permutation of fluorescent proteins provides a substrate for the design of molecular sensors. Here we describe a systematic exploration of permutation sites for mCherry and mKate using a tandem fusion template approach. Circular permutants retaining more than 60% (mCherry) and 90% (mKate) b...

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Detalles Bibliográficos
Autores principales: Shui, Bo, Wang, Qi, Lee, Frank, Byrnes, Laura J., Chudakov, Dmitry M., Lukyanov, Sergey A., Sondermann, Holger, Kotlikoff, Michael I.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3103546/
https://www.ncbi.nlm.nih.gov/pubmed/21647365
http://dx.doi.org/10.1371/journal.pone.0020505