Heterologous Overexpression and Mutagenesis of the Human Bile Salt Export Pump (ABCB11) Using DREAM (Directed REcombination-Assisted Mutagenesis)

Homologous recombination in Saccharomyces cerevisiae is a well-studied process. Here, we describe a yeast-recombination-based approach to construct and mutate plasmids containing the cDNA of the human bile salt export pump (BSEP) that has been shown to be unstable in E. coli. Using this approach, we...

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Detalles Bibliográficos
Autores principales: Stindt, Jan, Ellinger, Philipp, Stross, Claudia, Keitel, Verena, Häussinger, Dieter, Smits, Sander H. J., Kubitz, Ralf, Schmitt, Lutz
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105083/
https://www.ncbi.nlm.nih.gov/pubmed/21655228
http://dx.doi.org/10.1371/journal.pone.0020562

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105083/
https://www.ncbi.nlm.nih.gov/pubmed/21655228
http://dx.doi.org/10.1371/journal.pone.0020562

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