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Homolog-specific PCR primer design for profiling splice variants

To study functional diversity of proteins encoded from a single gene, it is important to distinguish the expression levels among the alternatively spliced variants. A variant-specific primer pair is required to amplify each alternatively spliced variant individually. For this purpose, we developed a...

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Detalles Bibliográficos
Autores principales: Srivastava, Gyan Prakash, Hanumappa, Mamatha, Kushwaha, Garima, Nguyen, Henry T., Xu, Dong
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105385/
https://www.ncbi.nlm.nih.gov/pubmed/21415011
http://dx.doi.org/10.1093/nar/gkr127