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Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis

Mammalian cells exhibit a frequent pericentrosomal Golgi ribbon organization. In this paper, we show that two AKAP450 N-terminal fragments, both containing the Golgi-binding GM130-interacting domain of AKAP450, dissociated endogenous AKAP450 from the Golgi and inhibited microtubule (MT) nucleation a...

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Autores principales: Hurtado, Lidia, Caballero, Cristina, Gavilan, Maria P., Cardenas, Jesus, Bornens, Michel, Rios, Rosa M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105543/
https://www.ncbi.nlm.nih.gov/pubmed/21606206
http://dx.doi.org/10.1083/jcb.201011014
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author Hurtado, Lidia
Caballero, Cristina
Gavilan, Maria P.
Cardenas, Jesus
Bornens, Michel
Rios, Rosa M.
author_facet Hurtado, Lidia
Caballero, Cristina
Gavilan, Maria P.
Cardenas, Jesus
Bornens, Michel
Rios, Rosa M.
author_sort Hurtado, Lidia
collection PubMed
description Mammalian cells exhibit a frequent pericentrosomal Golgi ribbon organization. In this paper, we show that two AKAP450 N-terminal fragments, both containing the Golgi-binding GM130-interacting domain of AKAP450, dissociated endogenous AKAP450 from the Golgi and inhibited microtubule (MT) nucleation at the Golgi without interfering with centrosomal activity. These two fragments had, however, strikingly different effects on both Golgi apparatus (GA) integrity and positioning, whereas the short fragment induced GA circularization and ribbon fragmentation, the large construct that encompasses an additional p150glued/MT-binding domain induced separation of the Golgi ribbon from the centrosome. These distinct phenotypes arose by specific interference of each fragment with either Golgi-dependent or centrosome-dependent stages of Golgi assembly. We could thus demonstrate that breaking the polarity axis by perturbing GA positioning has a more dramatic effect on directional cell migration than disrupting the Golgi ribbon. Both features, however, were required for ciliogenesis. We thus identified AKAP450 as a key determinant of pericentrosomal Golgi ribbon integrity, positioning, and function in mammalian cells.
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spelling pubmed-31055432011-11-30 Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis Hurtado, Lidia Caballero, Cristina Gavilan, Maria P. Cardenas, Jesus Bornens, Michel Rios, Rosa M. J Cell Biol Research Articles Mammalian cells exhibit a frequent pericentrosomal Golgi ribbon organization. In this paper, we show that two AKAP450 N-terminal fragments, both containing the Golgi-binding GM130-interacting domain of AKAP450, dissociated endogenous AKAP450 from the Golgi and inhibited microtubule (MT) nucleation at the Golgi without interfering with centrosomal activity. These two fragments had, however, strikingly different effects on both Golgi apparatus (GA) integrity and positioning, whereas the short fragment induced GA circularization and ribbon fragmentation, the large construct that encompasses an additional p150glued/MT-binding domain induced separation of the Golgi ribbon from the centrosome. These distinct phenotypes arose by specific interference of each fragment with either Golgi-dependent or centrosome-dependent stages of Golgi assembly. We could thus demonstrate that breaking the polarity axis by perturbing GA positioning has a more dramatic effect on directional cell migration than disrupting the Golgi ribbon. Both features, however, were required for ciliogenesis. We thus identified AKAP450 as a key determinant of pericentrosomal Golgi ribbon integrity, positioning, and function in mammalian cells. The Rockefeller University Press 2011-05-30 /pmc/articles/PMC3105543/ /pubmed/21606206 http://dx.doi.org/10.1083/jcb.201011014 Text en © 2011 Hurtado et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Hurtado, Lidia
Caballero, Cristina
Gavilan, Maria P.
Cardenas, Jesus
Bornens, Michel
Rios, Rosa M.
Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis
title Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis
title_full Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis
title_fullStr Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis
title_full_unstemmed Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis
title_short Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis
title_sort disconnecting the golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105543/
https://www.ncbi.nlm.nih.gov/pubmed/21606206
http://dx.doi.org/10.1083/jcb.201011014
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