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The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: Providing guidelines for Quality Assurance when working on membranes

BACKGROUND: The classical spoligotyping technique, relying on membrane reverse line-blot hybridization of the spacers of the Mycobacterium tuberculosis CRISPR locus, is used world-wide (598 references in Pubmed on April 8th, 2011). However, until now no inter-laboratory quality control study had bee...

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Detalles Bibliográficos
Autores principales: Abadia, Edgar, Zhang, Jian, Ritacco, Viviana, Kremer, Kristin, Ruimy, Raymond, Rigouts, Leen, Gomes, Harrison Magdinier, Elias, Atiná Ribeiro, Fauville-Dufaux, Maryse, Stoffels, Karolien, Rasolofo-Razanamparany, Voahangy, de Viedma, Darío Garcia, Herranz, Marta, Al-Hajoj, Sahal, Rastogi, Nalin, Garzelli, Carlo, Tortoli, Enrico, Suffys, Philip N, van Soolingen, Dick, Refrégier, Guislaine, Sola, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3107175/
https://www.ncbi.nlm.nih.gov/pubmed/21527037
http://dx.doi.org/10.1186/1471-2334-11-110