Quick and Clean Cloning: A Ligation-Independent Cloning Strategy for Selective Cloning of Specific PCR Products from Non-Specific Mixes

We have developed an efficient strategy for cloning of PCR products that contain an unknown region flanked by a known sequence. As with ligation-independent cloning, the strategy is based on homology between sequences present in both the vector and the insert. However, in contrast to ligation-indepe...

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Detalles Bibliográficos
Autores principales: Thieme, Frank, Engler, Carola, Kandzia, Romy, Marillonnet, Sylvestre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3107216/
https://www.ncbi.nlm.nih.gov/pubmed/21655102
http://dx.doi.org/10.1371/journal.pone.0020556

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3107216/
https://www.ncbi.nlm.nih.gov/pubmed/21655102
http://dx.doi.org/10.1371/journal.pone.0020556

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