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Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR
BACKGROUND: Accurate interpretation of quantitative PCR (qPCR) data requires normalization using constitutively expressed reference genes. Ribosomal RNA is often used as a reference gene for transcriptional studies in E. coli. However, the choice of reliable reference genes has not been systematical...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110127/ https://www.ncbi.nlm.nih.gov/pubmed/21513543 http://dx.doi.org/10.1186/1471-2199-12-18 |