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Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR

BACKGROUND: Accurate interpretation of quantitative PCR (qPCR) data requires normalization using constitutively expressed reference genes. Ribosomal RNA is often used as a reference gene for transcriptional studies in E. coli. However, the choice of reliable reference genes has not been systematical...

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Detalles Bibliográficos
Autores principales:	Zhou, Kang, Zhou, Lihan, Lim, Qing 'En, Zou, Ruiyang, Stephanopoulos, Gregory, Too, Heng-Phon
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110127/ https://www.ncbi.nlm.nih.gov/pubmed/21513543 http://dx.doi.org/10.1186/1471-2199-12-18

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