Fluoride-Cleavable, Fluorescently Labelled Reversible Terminators: Synthesis and Use in Primer Extension

Fluorescent 2′-deoxynucleotides containing a protecting group at the 3′-O-position are reversible terminators that enable array-based DNA sequencing-by-synthesis (SBS) approaches. Herein, we describe the synthesis and full characterisation of four reversible terminators bearing a 3′-blocking moiety...

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Detalles Bibliográficos
Autores principales: Knapp, Diana C, Serva, Saulius, D'Onofrio, Jennifer, Keller, Angelika, Lubys, Arvydas, Kurg, Ants, Remm, Maido, Engels, Joachim W
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2011
Materias:
Full Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110862/
https://www.ncbi.nlm.nih.gov/pubmed/21294195
http://dx.doi.org/10.1002/chem.201001952

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110862/
https://www.ncbi.nlm.nih.gov/pubmed/21294195
http://dx.doi.org/10.1002/chem.201001952

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