Ancient Microbes from Halite Fluid Inclusions: Optimized Surface Sterilization and DNA Extraction

Fluid inclusions in evaporite minerals (halite, gypsum, etc.) potentially preserve genetic records of microbial diversity and changing environmental conditions of Earth's hydrosphere for nearly one billion years. Here we describe a robust protocol for surface sterilization and retrieval of DNA...

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Detalles Bibliográficos
Autores principales: Sankaranarayanan, Krithivasan, Timofeeff, Michael N., Spathis, Rita, Lowenstein, Tim K., Lum, J. Koji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111412/
https://www.ncbi.nlm.nih.gov/pubmed/21694765
http://dx.doi.org/10.1371/journal.pone.0020683

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111412/
https://www.ncbi.nlm.nih.gov/pubmed/21694765
http://dx.doi.org/10.1371/journal.pone.0020683

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