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Ancient Microbes from Halite Fluid Inclusions: Optimized Surface Sterilization and DNA Extraction
Fluid inclusions in evaporite minerals (halite, gypsum, etc.) potentially preserve genetic records of microbial diversity and changing environmental conditions of Earth's hydrosphere for nearly one billion years. Here we describe a robust protocol for surface sterilization and retrieval of DNA...
Autores principales: | Sankaranarayanan, Krithivasan, Timofeeff, Michael N., Spathis, Rita, Lowenstein, Tim K., Lum, J. Koji |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111412/ https://www.ncbi.nlm.nih.gov/pubmed/21694765 http://dx.doi.org/10.1371/journal.pone.0020683 |
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