Phos-Tag-Based Analysis of Myosin Regulatory Light Chain Phosphorylation in Human Uterine Myocytes

BACKGROUND: The ‘phosphate-binding tag’ (phos-tag) reagent enables separation of phospho-proteins during SDS-PAGE by impeding migration proportional to their phosphorylation stoichiometry. Western blotting can then be used to detect and quantify the bands corresponding to the phospho-states of a tar...

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Detalles Bibliográficos
Autores principales: Aguilar, Hector N., Tracey, Curtis N., Tsang, Siu Cheung F., McGinnis, Justin M., Mitchell, Bryan F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111472/
https://www.ncbi.nlm.nih.gov/pubmed/21695279
http://dx.doi.org/10.1371/journal.pone.0020903

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111472/
https://www.ncbi.nlm.nih.gov/pubmed/21695279
http://dx.doi.org/10.1371/journal.pone.0020903

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