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Echium acanthocarpum hairy root cultures, a suitable system for polyunsaturated fatty acid studies and production
BACKGROUND: The therapeutic and health promoting role of highly unsaturated fatty acids (HUFAs) from fish, i.e. eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) are well known. These same benefits may however be shared by some of their precursors, the polyunsaturated fatt...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3114721/ https://www.ncbi.nlm.nih.gov/pubmed/21524311 http://dx.doi.org/10.1186/1472-6750-11-42 |
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author | Cequier-Sánchez, Elena Rodríguez, Covadonga Dorta-Guerra, Roberto Ravelo, Ángel G Zárate, Rafael |
author_facet | Cequier-Sánchez, Elena Rodríguez, Covadonga Dorta-Guerra, Roberto Ravelo, Ángel G Zárate, Rafael |
author_sort | Cequier-Sánchez, Elena |
collection | PubMed |
description | BACKGROUND: The therapeutic and health promoting role of highly unsaturated fatty acids (HUFAs) from fish, i.e. eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) are well known. These same benefits may however be shared by some of their precursors, the polyunsaturated fatty acids (PUFAs), such as stearidonic acid (SDA, 18:4 n-3). In order to obtain alternative sources for the large-scale production of PUFAs, new searches are being conducted focusing on higher plants oils which can contain these n-3 and n-6 C18 precursors, i.e. SDA and GLA (18:3n-6, γ-linolenic acid). RESULTS: The establishment of the novel Echium acanthocarpum hairy root cultures represents a powerful tool in order to research the accumulation and metabolism of fatty acids (FAs) in a plant particularly rich in GLA and SDA. Furthermore, this study constitutes the first example of a Boraginaceae species hairy root induction and establishment for FA studies and production. The dominant PUFAs, 18:2n-6 (LA, linoleic acid) and 18:3n-6 (GLA), accounted for about 50% of total FAs obtained, while the n-3 PUFAs, 18:3n-3 (ALA, α-linolenic acid) and 18:4n-3 (SDA), represented approximately 5% of the total. Production of FAs did not parallel hairy root growth, and the optimal productivity was always associated with the highest biomass density during the culture period. Assuming a compromise between FA production and hairy root biomass, it was determined that sampling times 4 and 5 gave the most useful FA yields. Total lipid amounts were in general comparable between the different hairy root lines (29.75 and 60.95 mg/g DW), with the major lipid classes being triacylglycerols. The FAs were chiefly stored in the hairy roots with very minute amounts being released into the liquid nutrient medium. CONCLUSIONS: The novel results presented here show the utility and high potential of E. acanthocarpum hairy roots. They are capable of biosynthesizing and accumulating a large range of polyunsaturated FAs, including the target GLA and SDA fatty acids in appreciable quantities. |
format | Online Article Text |
id | pubmed-3114721 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31147212011-06-15 Echium acanthocarpum hairy root cultures, a suitable system for polyunsaturated fatty acid studies and production Cequier-Sánchez, Elena Rodríguez, Covadonga Dorta-Guerra, Roberto Ravelo, Ángel G Zárate, Rafael BMC Biotechnol Research Article BACKGROUND: The therapeutic and health promoting role of highly unsaturated fatty acids (HUFAs) from fish, i.e. eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) are well known. These same benefits may however be shared by some of their precursors, the polyunsaturated fatty acids (PUFAs), such as stearidonic acid (SDA, 18:4 n-3). In order to obtain alternative sources for the large-scale production of PUFAs, new searches are being conducted focusing on higher plants oils which can contain these n-3 and n-6 C18 precursors, i.e. SDA and GLA (18:3n-6, γ-linolenic acid). RESULTS: The establishment of the novel Echium acanthocarpum hairy root cultures represents a powerful tool in order to research the accumulation and metabolism of fatty acids (FAs) in a plant particularly rich in GLA and SDA. Furthermore, this study constitutes the first example of a Boraginaceae species hairy root induction and establishment for FA studies and production. The dominant PUFAs, 18:2n-6 (LA, linoleic acid) and 18:3n-6 (GLA), accounted for about 50% of total FAs obtained, while the n-3 PUFAs, 18:3n-3 (ALA, α-linolenic acid) and 18:4n-3 (SDA), represented approximately 5% of the total. Production of FAs did not parallel hairy root growth, and the optimal productivity was always associated with the highest biomass density during the culture period. Assuming a compromise between FA production and hairy root biomass, it was determined that sampling times 4 and 5 gave the most useful FA yields. Total lipid amounts were in general comparable between the different hairy root lines (29.75 and 60.95 mg/g DW), with the major lipid classes being triacylglycerols. The FAs were chiefly stored in the hairy roots with very minute amounts being released into the liquid nutrient medium. CONCLUSIONS: The novel results presented here show the utility and high potential of E. acanthocarpum hairy roots. They are capable of biosynthesizing and accumulating a large range of polyunsaturated FAs, including the target GLA and SDA fatty acids in appreciable quantities. BioMed Central 2011-04-27 /pmc/articles/PMC3114721/ /pubmed/21524311 http://dx.doi.org/10.1186/1472-6750-11-42 Text en Copyright ©2011 Cequier-Sánchez et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cequier-Sánchez, Elena Rodríguez, Covadonga Dorta-Guerra, Roberto Ravelo, Ángel G Zárate, Rafael Echium acanthocarpum hairy root cultures, a suitable system for polyunsaturated fatty acid studies and production |
title | Echium acanthocarpum hairy root cultures, a suitable system for polyunsaturated fatty acid studies and production |
title_full | Echium acanthocarpum hairy root cultures, a suitable system for polyunsaturated fatty acid studies and production |
title_fullStr | Echium acanthocarpum hairy root cultures, a suitable system for polyunsaturated fatty acid studies and production |
title_full_unstemmed | Echium acanthocarpum hairy root cultures, a suitable system for polyunsaturated fatty acid studies and production |
title_short | Echium acanthocarpum hairy root cultures, a suitable system for polyunsaturated fatty acid studies and production |
title_sort | echium acanthocarpum hairy root cultures, a suitable system for polyunsaturated fatty acid studies and production |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3114721/ https://www.ncbi.nlm.nih.gov/pubmed/21524311 http://dx.doi.org/10.1186/1472-6750-11-42 |
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