Refolding by High Pressure of a Toxin Containing Seven Disulfide Bonds: Bothropstoxin-1 from Bothrops jararacussu

Aggregation is a serious obstacle for recovery of biologically active heterologous proteins from inclusion bodies (IBs) produced by recombinant bacteria. E. coli transformed with a vector containing the cDNA for Bothropstoxin-1 (BthTx-1) expressed the recombinant product as IBs. In order to obtain t...

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Detalles Bibliográficos
Autores principales: Balduino, Keli N., Spencer, Patrick J., Malavasi, Natalia V., Chura-Chambi, Rosa M., Lemke, Laura S., Morganti, Ligia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Humana Press Inc 2010
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3115051/
https://www.ncbi.nlm.nih.gov/pubmed/21181456
http://dx.doi.org/10.1007/s12033-010-9363-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3115051/
https://www.ncbi.nlm.nih.gov/pubmed/21181456
http://dx.doi.org/10.1007/s12033-010-9363-5

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