Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris

BACKGROUND: When performing functional and structural studies, large quantities of pure protein are desired. Most membrane proteins are however not abundantly expressed in their native tissues, which in general rules out purification from natural sources. Heterologous expression, especially of eukar...

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Detalles Bibliográficos
Autores principales: Nordén, Kristina, Agemark, Maria, Danielson, Jonas ÅH, Alexandersson, Erik, Kjellbom, Per, Johanson, Urban
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118338/
https://www.ncbi.nlm.nih.gov/pubmed/21569231
http://dx.doi.org/10.1186/1472-6750-11-47

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118338/
https://www.ncbi.nlm.nih.gov/pubmed/21569231
http://dx.doi.org/10.1186/1472-6750-11-47

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