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Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris
BACKGROUND: When performing functional and structural studies, large quantities of pure protein are desired. Most membrane proteins are however not abundantly expressed in their native tissues, which in general rules out purification from natural sources. Heterologous expression, especially of eukar...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118338/ https://www.ncbi.nlm.nih.gov/pubmed/21569231 http://dx.doi.org/10.1186/1472-6750-11-47 |