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Coronaviruses: Propagation, Quantification, Storage, and Construction of Recombinant Mouse Hepatitis Virus

The focus of this protocol is mouse hepatitis virus (MHV), with occasional references to other coronaviruses. Many of these protocols can be easily adapted to other coronaviruses. Protocols for propagating MHV in DBT and 17CL‐1 cells; the storage and titration of viral stocks; purification of MHV on...

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Detalles Bibliográficos
Autores principales: Leibowitz, Julian, Kaufman, Gili, Liu, Pinghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3119930/
https://www.ncbi.nlm.nih.gov/pubmed/21538303
http://dx.doi.org/10.1002/9780471729259.mc15e01s21