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Strain engineering for improved expression of recombinant proteins in bacteria

Protein expression in Escherichia coli represents the most facile approach for the preparation of non-glycosylated proteins for analytical and preparative purposes. So far, the optimization of recombinant expression has largely remained a matter of trial and error and has relied upon varying paramet...

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Detalles Bibliográficos
Autores principales: Makino, Tomohiro, Skretas, Georgios, Georgiou, George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3120638/
https://www.ncbi.nlm.nih.gov/pubmed/21569582
http://dx.doi.org/10.1186/1475-2859-10-32