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Strain engineering for improved expression of recombinant proteins in bacteria
Protein expression in Escherichia coli represents the most facile approach for the preparation of non-glycosylated proteins for analytical and preparative purposes. So far, the optimization of recombinant expression has largely remained a matter of trial and error and has relied upon varying paramet...
Autores principales: | Makino, Tomohiro, Skretas, Georgios, Georgiou, George |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3120638/ https://www.ncbi.nlm.nih.gov/pubmed/21569582 http://dx.doi.org/10.1186/1475-2859-10-32 |
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