The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing

BACKGROUND: The use of RNAi to analyse gene function in vitro is now widely applied in biological research. However, several difficulties are associated with its use in vivo, mainly relating to inefficient delivery and non-specific effects of short RNA duplexes in animal models. The latter can lead...

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Detalles Bibliográficos
Autores principales: Herbert, Mike, Coppieters, Natacha, Lasham, Annette, Cao, Helen, Reid, Glen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3123217/
https://www.ncbi.nlm.nih.gov/pubmed/21612654
http://dx.doi.org/10.1186/1756-0500-4-148

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3123217/
https://www.ncbi.nlm.nih.gov/pubmed/21612654
http://dx.doi.org/10.1186/1756-0500-4-148

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