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The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing
BACKGROUND: The use of RNAi to analyse gene function in vitro is now widely applied in biological research. However, several difficulties are associated with its use in vivo, mainly relating to inefficient delivery and non-specific effects of short RNA duplexes in animal models. The latter can lead...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3123217/ https://www.ncbi.nlm.nih.gov/pubmed/21612654 http://dx.doi.org/10.1186/1756-0500-4-148 |