Streamlined protein expression and purification using cleavable self-aggregating tags

BACKGROUND: Recombinant protein expression and purification remains a fundamental issue for biotechnology. Recently we found that two short self-assembling amphipathic peptides 18A (EWLKAFYEKVLEKLKELF) and ELK16 (LELELKLKLELELKLK) can induce the formation of active protein aggregates in Escherichia...

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Detalles Bibliográficos
Autores principales: Xing, Lei, Wu, Wei, Zhou, Bihong, Lin, Zhanglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124420/
https://www.ncbi.nlm.nih.gov/pubmed/21631955
http://dx.doi.org/10.1186/1475-2859-10-42

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124420/
https://www.ncbi.nlm.nih.gov/pubmed/21631955
http://dx.doi.org/10.1186/1475-2859-10-42

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