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Streamlined protein expression and purification using cleavable self-aggregating tags

BACKGROUND: Recombinant protein expression and purification remains a fundamental issue for biotechnology. Recently we found that two short self-assembling amphipathic peptides 18A (EWLKAFYEKVLEKLKELF) and ELK16 (LELELKLKLELELKLK) can induce the formation of active protein aggregates in Escherichia...

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Detalles Bibliográficos
Autores principales:	Xing, Lei, Wu, Wei, Zhou, Bihong, Lin, Zhanglin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3124420/ https://www.ncbi.nlm.nih.gov/pubmed/21631955 http://dx.doi.org/10.1186/1475-2859-10-42

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