Homologous RNA recombination allows efficient introduction of site-specific mutations into the genome of coronavirus MHV-A59 via synthetic co-replicating RNAs.

We describe a novel strategy to site-specifically mutagenize the genome of an RNA virus by exploiting homologous RNA recombination between synthetic defective interfering (DI) RNA and the viral RNA. The construction of a full-length cDNA clone, pMIDI, of a DI RNA of coronavirus MHV strain A59 was re...

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Detalles Bibliográficos
Autores principales: van der Most, R G, Heijnen, L, Spaan, W J, de Groot, R J
Formato: Texto
Lenguaje:English
Publicado: 1992
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC312492/
https://www.ncbi.nlm.nih.gov/pubmed/1630909

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC312492/
https://www.ncbi.nlm.nih.gov/pubmed/1630909

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