A Ribosomal Misincorporation of Lys for Arg in Human Triosephosphate Isomerase Expressed in Escherichia coli Gives Rise to Two Protein Populations

We previously observed that human homodimeric triosephosphate isomerase (HsTIM) expressed in Escherichia coli and purified to apparent homogeneity exhibits two significantly different thermal transitions. A detailed exploration of the phenomenon showed that the preparations contain two proteins; one...

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Detalles Bibliográficos
Autores principales: Aguirre, Beatriz, Costas, Miguel, Cabrera, Nallely, Mendoza-Hernández, Guillermo, Helseth, Donald L., Fernández, Paulette, Tuena de Gómez-Puyou, Marietta, Pérez-Montfort, Ruy, Torres-Larios, Alfredo, Gómez Puyou, Armando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3125179/
https://www.ncbi.nlm.nih.gov/pubmed/21738601
http://dx.doi.org/10.1371/journal.pone.0021035

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3125179/
https://www.ncbi.nlm.nih.gov/pubmed/21738601
http://dx.doi.org/10.1371/journal.pone.0021035

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