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A Ribosomal Misincorporation of Lys for Arg in Human Triosephosphate Isomerase Expressed in Escherichia coli Gives Rise to Two Protein Populations
We previously observed that human homodimeric triosephosphate isomerase (HsTIM) expressed in Escherichia coli and purified to apparent homogeneity exhibits two significantly different thermal transitions. A detailed exploration of the phenomenon showed that the preparations contain two proteins; one...
Autores principales: | Aguirre, Beatriz, Costas, Miguel, Cabrera, Nallely, Mendoza-Hernández, Guillermo, Helseth, Donald L., Fernández, Paulette, Tuena de Gómez-Puyou, Marietta, Pérez-Montfort, Ruy, Torres-Larios, Alfredo, Gómez Puyou, Armando |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3125179/ https://www.ncbi.nlm.nih.gov/pubmed/21738601 http://dx.doi.org/10.1371/journal.pone.0021035 |
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