Imaging individual mRNA molecules using multiple singly labeled probes

We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot via fluorescence microscopy. We demonstrate si...

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Detalles Bibliográficos
Autores principales: Raj, Arjun, van den Bogaard, Patrick, Rifkin, Scott A, van Oudenaarden, Alexander, Tyagi, Sanjay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3126653/
https://www.ncbi.nlm.nih.gov/pubmed/18806792
http://dx.doi.org/10.1038/nmeth.1253

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3126653/
https://www.ncbi.nlm.nih.gov/pubmed/18806792
http://dx.doi.org/10.1038/nmeth.1253

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