Imaging individual mRNA molecules using multiple singly labeled probes

We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot via fluorescence microscopy. We demonstrate si...

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Detalles Bibliográficos
Autores principales: Raj, Arjun, van den Bogaard, Patrick, Rifkin, Scott A, van Oudenaarden, Alexander, Tyagi, Sanjay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3126653/
https://www.ncbi.nlm.nih.gov/pubmed/18806792
http://dx.doi.org/10.1038/nmeth.1253
Descripción
Sumario:We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot via fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, mammalian cell lines and neurons.

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