Imaging individual mRNA molecules using multiple singly labeled probes

We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot via fluorescence microscopy. We demonstrate si...

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Detalles Bibliográficos
Autores principales: Raj, Arjun, van den Bogaard, Patrick, Rifkin, Scott A, van Oudenaarden, Alexander, Tyagi, Sanjay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3126653/
https://www.ncbi.nlm.nih.gov/pubmed/18806792
http://dx.doi.org/10.1038/nmeth.1253
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author Raj, Arjun
van den Bogaard, Patrick
Rifkin, Scott A
van Oudenaarden, Alexander
Tyagi, Sanjay
author_facet Raj, Arjun
van den Bogaard, Patrick
Rifkin, Scott A
van Oudenaarden, Alexander
Tyagi, Sanjay
author_sort Raj, Arjun
collection PubMed
description We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot via fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, mammalian cell lines and neurons.
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spelling pubmed-31266532011-06-29 Imaging individual mRNA molecules using multiple singly labeled probes Raj, Arjun van den Bogaard, Patrick Rifkin, Scott A van Oudenaarden, Alexander Tyagi, Sanjay Nat Methods Article We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot via fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, mammalian cell lines and neurons. 2008-09-21 2008-10 /pmc/articles/PMC3126653/ /pubmed/18806792 http://dx.doi.org/10.1038/nmeth.1253 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Raj, Arjun
van den Bogaard, Patrick
Rifkin, Scott A
van Oudenaarden, Alexander
Tyagi, Sanjay
Imaging individual mRNA molecules using multiple singly labeled probes
title Imaging individual mRNA molecules using multiple singly labeled probes
title_full Imaging individual mRNA molecules using multiple singly labeled probes
title_fullStr Imaging individual mRNA molecules using multiple singly labeled probes
title_full_unstemmed Imaging individual mRNA molecules using multiple singly labeled probes
title_short Imaging individual mRNA molecules using multiple singly labeled probes
title_sort imaging individual mrna molecules using multiple singly labeled probes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3126653/
https://www.ncbi.nlm.nih.gov/pubmed/18806792
http://dx.doi.org/10.1038/nmeth.1253
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