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Imaging individual mRNA molecules using multiple singly labeled probes

We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot via fluorescence microscopy. We demonstrate si...

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Detalles Bibliográficos
Autores principales:	Raj, Arjun, van den Bogaard, Patrick, Rifkin, Scott A, van Oudenaarden, Alexander, Tyagi, Sanjay
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2008
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3126653/ https://www.ncbi.nlm.nih.gov/pubmed/18806792 http://dx.doi.org/10.1038/nmeth.1253

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