A post-labeling method for multiplexed and multicolored genotyping analysis of SSR, indel and SNP markers in single tube with bar-coded split tag (BStag)

BACKGROUND: Genotyping analysis using capillary DNA sequencing with fluorescently labeled primer pairs obtained by polymerase chain reaction (PCR) is widely used, but is expensive. The post-PCR labeling method using fluorescently labeled short oligonucleotides and nested PCR of the amplified product...

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Detalles Bibliográficos
Autores principales: Shimizu, Tokurou, Yano, Kanako
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3126724/
https://www.ncbi.nlm.nih.gov/pubmed/21615927
http://dx.doi.org/10.1186/1756-0500-4-161

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3126724/
https://www.ncbi.nlm.nih.gov/pubmed/21615927
http://dx.doi.org/10.1186/1756-0500-4-161

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