Target Region Selection Is a Critical Determinant of Community Fingerprints Generated by 16S Pyrosequencing

Pyrosequencing of 16S rRNA genes allows for in-depth characterization of complex microbial communities. Although it is known that primer selection can influence the profile of a community generated by sequencing, the extent and severity of this bias on deep-sequencing methodologies is not well eluci...

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Detalles Bibliográficos
Autores principales: Kumar, Purnima S., Brooker, Michael R., Dowd, Scot E., Camerlengo, Terry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3126800/
https://www.ncbi.nlm.nih.gov/pubmed/21738596
http://dx.doi.org/10.1371/journal.pone.0020956

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3126800/
https://www.ncbi.nlm.nih.gov/pubmed/21738596
http://dx.doi.org/10.1371/journal.pone.0020956

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