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Target Region Selection Is a Critical Determinant of Community Fingerprints Generated by 16S Pyrosequencing
Pyrosequencing of 16S rRNA genes allows for in-depth characterization of complex microbial communities. Although it is known that primer selection can influence the profile of a community generated by sequencing, the extent and severity of this bias on deep-sequencing methodologies is not well eluci...
Autores principales: | Kumar, Purnima S., Brooker, Michael R., Dowd, Scot E., Camerlengo, Terry |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3126800/ https://www.ncbi.nlm.nih.gov/pubmed/21738596 http://dx.doi.org/10.1371/journal.pone.0020956 |
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