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Optimized localization-analysis for single-molecule tracking and super-resolution microscopy
We optimally localize isolated fluorescent beads and molecules imaged as diffraction-limited spots, determine the orientation of molecules, and present reliable formulae for the precisions of various localization methods. For beads, theory and experimental data both show that unweighted least-square...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3127582/ https://www.ncbi.nlm.nih.gov/pubmed/20364147 http://dx.doi.org/10.1038/nmeth.1447 |