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Lentiviral Mediated Transgenesis by In Vivo Manipulation of Spermatogonial Stem Cells

This report describes a technique for the generation of transgenic mice by in vivo manipulation of spermatogonial stem cells with a high rate of success. Spermatogonial stem cells (SSCs) in pre-pubescent animals were infected in vivo with recombinant lentiviruses expressing EGFP-f and mated with nor...

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Detalles Bibliográficos
Autores principales: Sehgal, Lalit, Thorat, Rahul, Khapare, Nileema, Mukhopadhaya, Amitabha, Sawant, Mugdha, Dalal, Sorab N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131306/
https://www.ncbi.nlm.nih.gov/pubmed/21760937
http://dx.doi.org/10.1371/journal.pone.0021975