An Optimized Protocol for DNA Extraction from Wheat Seeds and Loop-Mediated Isothermal Amplification (LAMP) to Detect Fusarium graminearum Contamination of Wheat Grain

A simple, rapid, and efficient method for isolating genomic DNA from germinated seeds of wheat that is free from polysaccharides and polyphenols is reported. DNA was extracted, treated with RNase, measured and tested for completeness using agarose gel electrophoresis. DNA purification from wheat gra...

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Detalles Bibliográficos
Autores principales: Abd-Elsalam, Kamel, Bahkali, Ali, Moslem, Mohamed, Amin, Osama E., Niessen, Ludwig
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131572/
https://www.ncbi.nlm.nih.gov/pubmed/21747688
http://dx.doi.org/10.3390/ijms12063459

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131572/
https://www.ncbi.nlm.nih.gov/pubmed/21747688
http://dx.doi.org/10.3390/ijms12063459

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