Cargando…

Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing

Mammals lacking BLOC-3 have impaired formation of melanosomes, a type of lysosome-related organelle (LRO), and, in earlier work, we found that a subunit of the BLOC-3 complex inhibits loading of Argonaute (Ago) proteins with small ribonucleic acids (RNAs) in Drosophila melanogaster cells. Small RNAs...

Descripción completa

Detalles Bibliográficos
Autores principales: Harris, Dinari A., Kim, Kevin, Nakahara, Kenji, Vásquez-Doorman, Constanza, Carthew, Richard W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135410/
https://www.ncbi.nlm.nih.gov/pubmed/21746852
http://dx.doi.org/10.1083/jcb.201102021
_version_ 1782208100387782656
author Harris, Dinari A.
Kim, Kevin
Nakahara, Kenji
Vásquez-Doorman, Constanza
Carthew, Richard W.
author_facet Harris, Dinari A.
Kim, Kevin
Nakahara, Kenji
Vásquez-Doorman, Constanza
Carthew, Richard W.
author_sort Harris, Dinari A.
collection PubMed
description Mammals lacking BLOC-3 have impaired formation of melanosomes, a type of lysosome-related organelle (LRO), and, in earlier work, we found that a subunit of the BLOC-3 complex inhibits loading of Argonaute (Ago) proteins with small ribonucleic acids (RNAs) in Drosophila melanogaster cells. Small RNAs such as small interfering RNAs (siRNAs) direct Ago proteins to repress the stability of messenger RNA transcripts. In this paper, we show that BLOC-3 is required for biogenesis of Drosophila LROs called pigment granules. Other complexes that sort cargo to pigment LROs also negatively regulate siRNA activity. However, regulation is not obligately linked to biogenesis of LROs but instead to specific cargo-sorting processes. Negative regulation is also not linked to sorting into all LROs but only a specific class of pigment LRO. Thus, regulation of siRNA activity is tied to sorting of specific types of cargo to particular LROs.
format Online
Article
Text
id pubmed-3135410
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-31354102012-01-11 Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing Harris, Dinari A. Kim, Kevin Nakahara, Kenji Vásquez-Doorman, Constanza Carthew, Richard W. J Cell Biol Research Articles Mammals lacking BLOC-3 have impaired formation of melanosomes, a type of lysosome-related organelle (LRO), and, in earlier work, we found that a subunit of the BLOC-3 complex inhibits loading of Argonaute (Ago) proteins with small ribonucleic acids (RNAs) in Drosophila melanogaster cells. Small RNAs such as small interfering RNAs (siRNAs) direct Ago proteins to repress the stability of messenger RNA transcripts. In this paper, we show that BLOC-3 is required for biogenesis of Drosophila LROs called pigment granules. Other complexes that sort cargo to pigment LROs also negatively regulate siRNA activity. However, regulation is not obligately linked to biogenesis of LROs but instead to specific cargo-sorting processes. Negative regulation is also not linked to sorting into all LROs but only a specific class of pigment LRO. Thus, regulation of siRNA activity is tied to sorting of specific types of cargo to particular LROs. The Rockefeller University Press 2011-07-11 /pmc/articles/PMC3135410/ /pubmed/21746852 http://dx.doi.org/10.1083/jcb.201102021 Text en © 2011 Harris et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Harris, Dinari A.
Kim, Kevin
Nakahara, Kenji
Vásquez-Doorman, Constanza
Carthew, Richard W.
Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing
title Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing
title_full Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing
title_fullStr Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing
title_full_unstemmed Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing
title_short Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing
title_sort cargo sorting to lysosome-related organelles regulates sirna-mediated gene silencing
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135410/
https://www.ncbi.nlm.nih.gov/pubmed/21746852
http://dx.doi.org/10.1083/jcb.201102021
work_keys_str_mv AT harrisdinaria cargosortingtolysosomerelatedorganellesregulatessirnamediatedgenesilencing
AT kimkevin cargosortingtolysosomerelatedorganellesregulatessirnamediatedgenesilencing
AT nakaharakenji cargosortingtolysosomerelatedorganellesregulatessirnamediatedgenesilencing
AT vasquezdoormanconstanza cargosortingtolysosomerelatedorganellesregulatessirnamediatedgenesilencing
AT carthewrichardw cargosortingtolysosomerelatedorganellesregulatessirnamediatedgenesilencing