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Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing
Mammals lacking BLOC-3 have impaired formation of melanosomes, a type of lysosome-related organelle (LRO), and, in earlier work, we found that a subunit of the BLOC-3 complex inhibits loading of Argonaute (Ago) proteins with small ribonucleic acids (RNAs) in Drosophila melanogaster cells. Small RNAs...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135410/ https://www.ncbi.nlm.nih.gov/pubmed/21746852 http://dx.doi.org/10.1083/jcb.201102021 |
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author | Harris, Dinari A. Kim, Kevin Nakahara, Kenji Vásquez-Doorman, Constanza Carthew, Richard W. |
author_facet | Harris, Dinari A. Kim, Kevin Nakahara, Kenji Vásquez-Doorman, Constanza Carthew, Richard W. |
author_sort | Harris, Dinari A. |
collection | PubMed |
description | Mammals lacking BLOC-3 have impaired formation of melanosomes, a type of lysosome-related organelle (LRO), and, in earlier work, we found that a subunit of the BLOC-3 complex inhibits loading of Argonaute (Ago) proteins with small ribonucleic acids (RNAs) in Drosophila melanogaster cells. Small RNAs such as small interfering RNAs (siRNAs) direct Ago proteins to repress the stability of messenger RNA transcripts. In this paper, we show that BLOC-3 is required for biogenesis of Drosophila LROs called pigment granules. Other complexes that sort cargo to pigment LROs also negatively regulate siRNA activity. However, regulation is not obligately linked to biogenesis of LROs but instead to specific cargo-sorting processes. Negative regulation is also not linked to sorting into all LROs but only a specific class of pigment LRO. Thus, regulation of siRNA activity is tied to sorting of specific types of cargo to particular LROs. |
format | Online Article Text |
id | pubmed-3135410 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-31354102012-01-11 Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing Harris, Dinari A. Kim, Kevin Nakahara, Kenji Vásquez-Doorman, Constanza Carthew, Richard W. J Cell Biol Research Articles Mammals lacking BLOC-3 have impaired formation of melanosomes, a type of lysosome-related organelle (LRO), and, in earlier work, we found that a subunit of the BLOC-3 complex inhibits loading of Argonaute (Ago) proteins with small ribonucleic acids (RNAs) in Drosophila melanogaster cells. Small RNAs such as small interfering RNAs (siRNAs) direct Ago proteins to repress the stability of messenger RNA transcripts. In this paper, we show that BLOC-3 is required for biogenesis of Drosophila LROs called pigment granules. Other complexes that sort cargo to pigment LROs also negatively regulate siRNA activity. However, regulation is not obligately linked to biogenesis of LROs but instead to specific cargo-sorting processes. Negative regulation is also not linked to sorting into all LROs but only a specific class of pigment LRO. Thus, regulation of siRNA activity is tied to sorting of specific types of cargo to particular LROs. The Rockefeller University Press 2011-07-11 /pmc/articles/PMC3135410/ /pubmed/21746852 http://dx.doi.org/10.1083/jcb.201102021 Text en © 2011 Harris et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Harris, Dinari A. Kim, Kevin Nakahara, Kenji Vásquez-Doorman, Constanza Carthew, Richard W. Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing |
title | Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing |
title_full | Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing |
title_fullStr | Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing |
title_full_unstemmed | Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing |
title_short | Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing |
title_sort | cargo sorting to lysosome-related organelles regulates sirna-mediated gene silencing |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135410/ https://www.ncbi.nlm.nih.gov/pubmed/21746852 http://dx.doi.org/10.1083/jcb.201102021 |
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